ABSTRACT:
Utilising an in vivo approach to induce urolithiasis with ethylene glycol, the research examines the anti-urolithiatic potential of a methanolic extract derived from the whole Hygrophila salicifolia plant. Over the course of 28 days, five groups of male Wistar albino rats were randomly assigned to different treatment groups. The first group was used as a control, the second group got a lithiatic agent, the third group had Cystone, and the fourth and fifth groups got two doses of the methanolic extract. From the urine samples, many parameters were analysed, including the amounts of calcium, creatinine, phosphate, magnesium, and oxalate. The development of calcium oxalate monohydrate (COM) crystals was significantly inhibited by the methanolic extract in comparison to the control and other extracts. A significance threshold of P<0.001 was used in the statistical study to validate the effectiveness.
Cite this article:
Santosh Bandhu Mohapatra, Ashish Sarkar. Evaluating the Anti-Ulolithiatic Potential of Methanolic Extract of Hygrophila salicifolia Using In vitro Method of Urolithiasis Induction. International Journal of Technology. 2024; 14(2):141-2. doi: 10.52711/2231-3915.2024.00021
Cite(Electronic):
Santosh Bandhu Mohapatra, Ashish Sarkar. Evaluating the Anti-Ulolithiatic Potential of Methanolic Extract of Hygrophila salicifolia Using In vitro Method of Urolithiasis Induction. International Journal of Technology. 2024; 14(2):141-2. doi: 10.52711/2231-3915.2024.00021 Available on: https://ijtonline.com/AbstractView.aspx?PID=2024-14-2-12
REFERENCES:
1. Kumar S, Singh R, Vasudeva N, Sharma S. Acute and chronic animal models for the evaluation of anti-diabetic agents. Cardiovasc Diabetol. 2022; 11(9):1-13.
2. Irchhaiya R., Kumar A, Gupta N, Yadav A, Kumar S, Gupta N, Kumar S, Gurjar H.Review on some medicinal plants with antidiabetic and analgesic activity.World Journal of Pharmacy and Pharmaceutical Sciences. 2019; 3(11): 163-196.
3. Haque MR, Islam M, Kuddus MD. In vitro and in vivo evaluation of pharmacological potential of Begonia barbata Wall. Future Journal of Pharmaceutical Sciences. 2020; 6:112.
4. Gurjar H, Irchhaiya R, Verma A. Review on some medicinal plants with antidiabetic activity. Journal of Drug Delivery and Therapeutics.2016; 6(2): 45-51.
5. Gupta R, Katariya P, Mathur M, Bajaj VK, Yadav S, Kamal R, Gupta RS. Antidiabetic and renoprotective activity of Momordica Dioica in diabetic rats. Diabetologia Croatica. 2021; 40(3): 81-88.
6. Barik R, Jain S, Qwatra D, Joshi A, Tripathi GS, Goyal R. Antidiabetic activity ofaqueous root extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats. Indian J Pharmacol. 2018; 40(1):19-22.
7. Lakhtakia R. The history of diabetes mellitus. Sultan Qaboos University MedicalJournal;13(3):368-370.
8. Moron MS, Joseph W, Depierre, Mannervik B. Levels of Glutathione, Glutathione Reductase and Glutathione S-Transferase Activities in Rat Lung and Liver. Biochimica et Biophysica Acta. 2022; 582: 67-78.
9. Nagarani G, Abirami A, Siddhuraju P. Food prospects and nutraceutical attributes of Momordica species: A potential tropical bio resources-A review. Food Science and Human Wellness. 2019; 3: 117-126.
10. OECD. OECD guidelines for testing of chemicals 423: acute oral toxicity-acute toxicclassmethod. Paris: OECD; 2021.
11. M. K. Bhatt and N. Shah. Anti-urolithiatic potential of Methanolic extract of Hygrophila salicifolia on ethylene glycol induced urolithiasis in Rats. International Journal of Pharmaceutical Sciences and Drug Research. 2016: 287–290
12. A. Makasana, V. Ranpariya, D. Desai, J. Mendpara, and V. Parekh, “Evaluation for the anti-urolithiatic activity of Launaea procumbens against ethylene glycol-induced renal calculi in rats,” Toxicology Reports, Vol. 1. Elsevier BV, pp. 46–52, 2014.
13. K. Ingale, P. Thakurdesai, and N. Vyawahare. Effect of Hygrophila spinosa in ethylene glycol induced nephrolithiasis in rats. Indian Journal of Pharmacology. 2012; 44(5): 639.
14. Dr. S. S. Nipate and R. R. Jori. Evaluation of Anti-Urolithiatic Activity of Ethanolic Extract of Delonix Regia Leaves in Experimental Animal. International Journal of Pharmaceutical Sciences Review and Research. 2022: 79–85.
15. S. Gopika, M. Nisha, E. G. Devi, A. R. Rajeswari, and R. Vasandhlakshmi. Evaluation of Antiurolithiatic Potential of Methanolic Stem Extract of Spermacocce articularis L.f.: An In vitro and In vivo Approach. Pharmacognosy Journal. 2024; 16(4): 770–778.
16. A. Pawar and N. Vyawahare. Anti-urolithiatic activity of standardized extract of Biophytum sensitivum against zinc disc implantation induced urolithiasis in rats. Journal of Advanced Pharmaceutical Technology and Research. 2015; 6(4): 176.
17. B. Kaleeswaran, S. Ramadevi, R. Murugesan, S. Srigopalram, T. Suman, and T. Balasubramanian. Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone). Journal of Traditional and Complementary Medicine. 2019; 9(1): 24–37.
18. N. El Menyiy et al. Litholytic Activities of Natural Bioactive Compounds and Their Mechanism Insights. Applied Sciences. 2021;11(18): 8702.
19. F. E. El oumari et al. Chemical Composition and Anti-Urolithiatic Activity of Extracts from Argania spinosa (L.) Skeels Press-Cake and Acacia senegal (L.) Willd. Molecules. 2022; 27(13): 3973.