2231-3915 (Online)
2231-3907 (Print)

Author(s): Vishal Jain, Sakshi Tiwari, Amber Vyas


DOI: 10.52711/2231-3915.2023.00013   

Address: Vishal Jain, Sakshi Tiwari, Amber Vyas*
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)
*Corresponding Author

Published In:   Volume - 13,      Issue - 2,     Year - 2023

Antibiotic resistance is increasing worldwide, especially among ocular pathogens and one of the major reasons for this severity is the formation of biofilm which causes antibacterial specialists like Ciprofloxacin HCl to be ineffective, but combining it with substances that inhibit quorum sensing, a process that leads towards the development of biofilm, such as Quercetin, in a single formulation is an effective way to treat these infections. Aiming to develop and approve a new analytical method for the simultaneous evaluation of ciprofloxacin HCl and quercetin in their mass powder, the current examination is expected to accomplish this. Two strategies—the simultaneous equation approach (I) and the absorbance ratio method (II)—were created and validated in accordance with ICH guidelines for specificity, selectivity, linearity, precision, and accuracy. Quercetin's and Ciprofloxacin HCl's absorbance maxima (?max) were determined to be at 327 nm and 271 nm, respectively, with coefficient correlation values of 0.999 and 0.997. Their isosbestic point was noticed at a maximum wavelength of 283 nm. Both Ciprofloxacin HCl and Quercetin demonstrated linearity in the focus range from 1 µg/ml to 10 µg/ml when absorbances were measured at any of the aforementioned levels. It was discovered that the developed processes were exact and precise with less than 2% relative standard deviation (%RSD). %Recovery studies were found to be 98.62-101.15% for Ciprofloxacin HCl at 271nm, 99.34-100.94% for Quercetin at 327nm, 99.63-104.62%, and 101.23-102.64% for Ciprofloxacin HCl and Quercetin respectively at their isosbestic point. Because it was discovered to be simple, rapid, specific, selective, linear, exact, and based on absorptivity measurements, the established UV Spectrophotometric technique can be used for in vitro depiction and contemporaneous assessment of Ciprofloxacin HCl and Quercetin.

Cite this article:
Vishal Jain, Sakshi Tiwari, Amber Vyas. UV- Spectrophotometric technique- based method development and validation for simultaneous estimation of Ciprofloxacin HCl and Quercetin in bulk powder. International Journal of Technology. 2023; 13(2)101-7: doi: 10.52711/2231-3915.2023.00013

Vishal Jain, Sakshi Tiwari, Amber Vyas. UV- Spectrophotometric technique- based method development and validation for simultaneous estimation of Ciprofloxacin HCl and Quercetin in bulk powder. International Journal of Technology. 2023; 13(2)101-7: doi: 10.52711/2231-3915.2023.00013   Available on:

1.    Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol. 2002;292(2):107-113. doi:10.1078/1438-4221-00196
2.    Diriba K, Kassa T, Alemu Y, Bekele S. In Vitro Biofilm Formation and Antibiotic Susceptibility Patterns of Bacteria from Suspected External Eye Infected Patients Attending Ophthalmology Clinic, Southwest Ethiopia. Int J Microbiol. 2020;2020. doi:10.1155/2020/8472395
3.    Vasudevan S, Swamy SS, Kaur G, Princy SA, Balamurugan P. Synergism between quorum sensing inhibitors and antibiotics: Combating the antibiotic resistance crisis. Biotechnol Appl Quor Sens Inhib. Published online 2018:209-225. doi:10.1007/978-981-10-9026-4_10
4.    Singh N, Romero M, Travanut A, et al. Dual bioresponsive antibiotic and quorum sensing inhibitor combination nanoparticles for treatment of: Pseudomonas aeruginosa biofilms in vitro and ex vivo. Biomater Sci. 2019;7(10):4099-4111. doi:10.1039/c9bm00773c
5.    Ouyang J, Sun F, Feng W, et al. Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa. J Appl Microbiol. 2016;120(4):966-974. doi:10.1111/jam.13073
6.    Singh R, Maithani M, Saraf SK, Saraf S, Gupta RC. Simultaneous Estimation of Ciprofloxacin Hydrochloride, Ofloxacin, Tinidazole and Ornidazole by Reverse Phase – High Performance Liquid Chromatography. Eurasian J Anal Chem. 2009;4(2):161-167.
7.    Baghel US, Nagar A, Pannu MS, Singh D, Yadav R. HPLC and HPTLC methods for simultaneous estimation of quercetin and curcumin in polyherbal formulation. Indian J Pharm Sci. 2017;79(2):197-203. doi:10.4172/pharmaceutical-sciences.1000217
8.    Fazil Ahmed M, Srinivasa Rao A. Simultaneous estimation of quercetin and rutin in ethanolic extract of Melia azedarach. Linn leaves by HPTLC method. Asian J Biomed Pharm Sci. 2013;3(21):56-59.
9.    Jain A, Lodhi S, Singhai AK. Simultaneous estimation of quercetin and rutin in Tephrosia purpurea Pers by high performance thin- layer chromatography. Asian J Tradit Med. 2009;4(3):104-109.
10.    Patel KB, Thula KC, Maheshwari DG. Original Research Paper stability indicating HPLC method for simultaneous Estimation of Ciprofloxacin and Phenylephrine in Pharmaceutical dosage form. Pharmacophore. 2014;5(2):262.
11.    Baldi A, Upendra S, Ashish B. Simultaneous Estimation of Quercetin and Silymarin: Method Development and Validation. Int J Pharm Biol Arch. 2013;4(3):527-531.
12.    Mohamed EH, Lotfy HM, Hegazy MA, Mowaka S. Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra. Chem Cent J. 2017;11(1):1-15. doi:10.1186/s13065-017-0270-8
13.    Sawant RL, Hadawale SD, Dhikale GK, Bansode CA, Tajane PS. Spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac from the combined capsule dosage form. Pharm Methods. 2011;2(3):193-197. doi:10.4103/2229-4708.90362
14.    Dhumal DM, Shirkhedkar AA, Nerkar PP, Surana SJ. Simultaneous estimation of moxifloxacin hydrochloride and dexamethasone sodium phosphate in bulk and in ophthalmic solution by RP-HPLC. J Chil Chem Soc. 2012;57(4):1344-1347. doi:10.4067/S0717-97072012000400003
15.    Bellah SF, Ullah S, Harris KK. IOSR Journal of Pharmacy paper , research paper publishing , where to publish ... IOSR J Pharm. 2012;6(3):44-48.
16.    ICH Q2B. ICH Q2B Guideline Validation of Analytical Procedures Methodology Comments for its application. Methodology.:71-76.
17.    Sireesha D, Monika ML, Bakshi V. Development and Validation of UV Spectrophotometric Method for The Simultaneous Estimation of Rosuvastatin and Ezetimibe in Pharmaceutical Dosage Form. Asian J Pharm Anal. 2017;7(3):135. doi:10.5958/2231-5675.2017.00021.7

Recomonded Articles:

International Journal of Technology (IJT) is an international, peer-reviewed journal, research journal aiming at promoting and publishing original high quality research in all disciplines of engineering sciences and technology...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231-3915 

Recent Articles